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The aim of this study is to assess the main features of the vegetation seasonality of the Amazon and Cerrado biomes in a gradient of four climatological classes, which were derived from CRU monthly precipitation dataset (1961-1990), being two classes (C5 and C4) of higher precipitation located in the Amazon biome and the other two (C3 and C2) of lower precipitation in the Cerrado biome.These all classes were cut for three longitudinal transects placed latitudinal and parallel between them in order to analyse the following vegetation seasonal parameters: start, end, length and amplitud of the vegetation growth cycle, as well as the integral of this cycle; derived from EVI time series data (2000 to 2012). Our results showed that in regions with higher rainfall, the vegetation seasonality is less pronounced than in region with lower rainfall. The transect analysis showed that the vegetation growth cycle parameters of T1 and T2 were similar between them and both (T1 and T2) were different of T3. The beginning and end of the vegetation growth cycle of C5 and C4 (Amazon) classes were not associated with the beginning and end of the rainy season, while the classes C3 and C2 (Cerrado) showed relationship between vegetation and rainfall. Vegetation growth cycle of classes C5 and C4 showed higher length, less amplitud and higher integral when compared to C3 and C2 classes that showed less length, higher amplitud and less integral. This means that the Cerrado vegetation has less productivity and higher seasonality than the Amazon Vegetation. This study showed that the vegetation growth cycle parameters were able to identify the main features of vegetation in each climatological region.